Journal: International Journal of Molecular Sciences

Article Title: Induction of Extrinsic Apoptotic Pathway in Pancreatic Cancer Cells by Apteranthes europaea Root Extract

doi: 10.3390/ijms262010221

Figure Lengend Snippet: A. europaea extract reduces cell viability of human pancreatic cells. PL45 ( A ) and Mia PaCa-2 ( B ) cells were treated with increasing concentrations of A. europaea (50–200 µg/mL) for 24, 48, and 72 h and cell viability was determined using XTT assay. Data represent an average of three independent experiments; per experiment n = 3–5 repeats (mean ± SE) and are expressed as a percentage of the respective vehicle untreated control. Statistical significance was determined by one-way ANOVA test for post hoc to compare between concentrations and two-way ANOVA for time and concentration interactions. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate statistically significant differences compared with the untreated control.

Article Snippet: The human pancreatic cancer cell lines Mia PaCa-2 (poorly differentiated) and PL45 (moderately to well-differentiated) (ATCC, Rockville, MD, USA) were maintained in DMEM medium, supplemented with 1% L-glutamine, 10% fetal bovine serum (FBS), 1% PenStrep (penicillin + streptomycin), and 1% sodium pyruvate (Biological industries, Beit HaEmek, Israel).

Techniques: XTT Assay, Control, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Induction of Extrinsic Apoptotic Pathway in Pancreatic Cancer Cells by Apteranthes europaea Root Extract

doi: 10.3390/ijms262010221

Figure Lengend Snippet: Measurement of apoptotic cells using Annexin V-FITC/PI double staining on PL45 ( a , b ) and Mia PaCa-2 ( c , d ) cells. In total, 10 6 PL-45 cells were treated with either 125, 150, or 175 µg/mL of extract of A. europaea for 48 and 72 h. A total of 10 6 Mia PaCa-2 cells were treated with either 150 or 175 µg/mL of the extract for 36 h and flow cytometric analysis of Annexin V-FITC/PI double staining was performed. In each plot ( a , c ), the lower left quadrant (Q3) represents viable cells, the upper left quadrant (Q1) indicates necrotic cells, the lower right quadrant (Q4) denotes early apoptotic cells, and the upper right quadrant (Q2) represents necrotic or late apoptosis cells. Data are presented as the mean ± SE of three independent experiments [mean (Q2 + Q4) ± SE] ( b , d ). Statistical significance was determined by a two-tailed Student’s t -test [treatment vs. control (untreated cells)] and is indicated as * p < 0.05, ** p < 0.01.

Article Snippet: The human pancreatic cancer cell lines Mia PaCa-2 (poorly differentiated) and PL45 (moderately to well-differentiated) (ATCC, Rockville, MD, USA) were maintained in DMEM medium, supplemented with 1% L-glutamine, 10% fetal bovine serum (FBS), 1% PenStrep (penicillin + streptomycin), and 1% sodium pyruvate (Biological industries, Beit HaEmek, Israel).

Techniques: Double Staining, Two Tailed Test, Control

Journal: International Journal of Molecular Sciences

Article Title: Induction of Extrinsic Apoptotic Pathway in Pancreatic Cancer Cells by Apteranthes europaea Root Extract

doi: 10.3390/ijms262010221

Figure Lengend Snippet: The IncuCyte live-cell analysis system used to study the effect of treatment with A. europaea extract on cell death in PL45 and Mia PaCa-2 cell lines. ( a1 ) Real-time plots for cell death measurements in PL45 cells exposed to 125, 150, and 175 µg/mL of A. europaea extract for 72 h. ( a2 , b2 ) Representative images at X20 objective, of IncuCyte quantification in PL45 ( a2 ) and Mia PaCa-2 ( b2 ) cells. Scale bar 200 µm. In red, cells labeled with PI; in green, cells labeled with Annexin V. ( b1 ) Real-time plots for cell death measurements in Mia PaCa-2 cells exposed to 125, 150, and 175 µg/mL of A. europaea extract for 36 h. Data are presented as mean ± SE of three independent experiments.

Article Snippet: The human pancreatic cancer cell lines Mia PaCa-2 (poorly differentiated) and PL45 (moderately to well-differentiated) (ATCC, Rockville, MD, USA) were maintained in DMEM medium, supplemented with 1% L-glutamine, 10% fetal bovine serum (FBS), 1% PenStrep (penicillin + streptomycin), and 1% sodium pyruvate (Biological industries, Beit HaEmek, Israel).

Techniques: Cell Analysis, Labeling

Journal: International Journal of Molecular Sciences

Article Title: Induction of Extrinsic Apoptotic Pathway in Pancreatic Cancer Cells by Apteranthes europaea Root Extract

doi: 10.3390/ijms262010221

Figure Lengend Snippet: Western blot analysis of the caspases and PARP. PL45 and Mia PaCa-2 cells were treated with A. europaea extract. Mia PaCa-2 cells were treated with 150 µg/mL of A. europaea extract for 36 h, while PL45 cells were treated with 175 µg/mL for 48 and 72 h. The level of caspase-8 ( a ), caspase-9 ( b ), caspase-3 ( c ), and PARP ( d ) was analyzed by Western blotting, where β-actin was used as the loading control.

Article Snippet: The human pancreatic cancer cell lines Mia PaCa-2 (poorly differentiated) and PL45 (moderately to well-differentiated) (ATCC, Rockville, MD, USA) were maintained in DMEM medium, supplemented with 1% L-glutamine, 10% fetal bovine serum (FBS), 1% PenStrep (penicillin + streptomycin), and 1% sodium pyruvate (Biological industries, Beit HaEmek, Israel).

Techniques: Western Blot, Control

Journal: Emerging Microbes & Infections

Article Title: Prevalence and characterization of influenza viruses in diverse species in Los Llanos, Colombia

doi: 10.1038/emi.2013.20

Figure Lengend Snippet: Replication of Colombian H5N2 viruses in vitro . MDCK ( A ) cells were infected at an MOI of 0.01, culture supernatants were collected at 0, 24 and 48 hpi, and virus was titrated as TCID 50 . ( B ) NHBE cells were maintained in culture at an air/liquid interface and infected at an MOI of 0.1. At 6, 24, 48 72 and 96 hpi, medium was added to the apical surface. Medium was collected after 30 min, and virus was titrated in triplicate as TCID 50 . Error bars represent the standard error of the mean. * P <0.05.

Article Snippet: Well-differentiated (transepithelial resistance >1000) primary normal human bronchial epithelial (NHBE) cells were purchased from MatTek Corp. (Ashland, MA, USA), maintained by daily washing with 0.9% sodium chloride, and incubated at the air-liquid interface at 37 °C and 5% CO 2 .

Techniques: In Vitro, Infection, Virus

Journal: Journal of Dentistry

Article Title: Efficacy and hazards of 425 nm oral cavity light dosing to inactivate SARS-CoV-2

doi: 10.1016/j.jdent.2022.104203

Figure Lengend Snippet: Assessment of acute light dose-induced cytotoxicity in well-differentiated human large airway or oral epithelial models . (A) AIR-100 (the MatTek EpiAirway model) was dosed with to 405 nm and 425 nm light at doses up to 255 J/cm 2 . As a positive control, a 120 J/cm 2 dose of UV (385 nm) light reduced viability to 4.3%. (B) ORL-200 (the MatTek EpiOral model) was dosed with 425 nm light at doses of up to 120 J/cm 2 . As a positive control, three minutes exposure to 3% hydrogen peroxide (H 2 O 2 ), a known mild irritant, reduced ORL-200 viability to 2.6% ( n = 5 replicates per dose; error bars represent standard deviation).

Article Snippet: Primary models derived from well-differentiated human large airway epithelial cells (AIR-100; MatTek Corporation, Ashland, MA, USA) or well-differentiated human buccal epithelial cells (ORL-200; MatTek Corporation, Ashland, MA, USA) were acquired, revived, and stored as previously described .

Techniques: Positive Control, Standard Deviation

Journal: Journal of Dentistry

Article Title: Efficacy and hazards of 425 nm oral cavity light dosing to inactivate SARS-CoV-2

doi: 10.1016/j.jdent.2022.104203

Figure Lengend Snippet: Assessment of acute light dose-induced cytotoxicity in well-differentiated human large airway or oral epithelial models . (A) AIR-100 (the MatTek EpiAirway model) was dosed with to 405 nm and 425 nm light at doses up to 255 J/cm 2 . As a positive control, a 120 J/cm 2 dose of UV (385 nm) light reduced viability to 4.3%. (B) ORL-200 (the MatTek EpiOral model) was dosed with 425 nm light at doses of up to 120 J/cm 2 . As a positive control, three minutes exposure to 3% hydrogen peroxide (H 2 O 2 ), a known mild irritant, reduced ORL-200 viability to 2.6% ( n = 5 replicates per dose; error bars represent standard deviation).

Article Snippet: Primary models derived from well-differentiated human large airway epithelial cells (AIR-100; MatTek Corporation, Ashland, MA, USA) or well-differentiated human buccal epithelial cells (ORL-200; MatTek Corporation, Ashland, MA, USA) were acquired, revived, and stored as previously described .

Techniques: Positive Control, Standard Deviation

Journal: Cancer letters

Article Title: Sensitivity of human prostate cancer cells to chemotherapeutic drugs depends on EndoG expression regulated by promoter methylation

doi: 10.1016/j.canlet.2008.04.053

Figure Lengend Snippet: Methylation of EndoG promoter/exon1 region in human prostate cancer cell lines. (A) EndoG promoter/exon1 DNA methylation measured by the McrBC-PCR screening method. (B) Quantification by densitometry. Optical density of 22Rv1 cells was considered 100%. n = 3 per group, * p <0.05 compared to 22Rv1 cells.

Article Snippet: Human prostate cancer cell lines, including well-differentiated, 22Rv1 (ATCC # CRL-2505) and LNCaP cells (ATCC # CRL-1740), and poorly differentiated PC-3 cells (ATCC #CRL-1435) were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Methylation, DNA Methylation Assay

Journal: Cancer letters

Article Title: Sensitivity of human prostate cancer cells to chemotherapeutic drugs depends on EndoG expression regulated by promoter methylation

doi: 10.1016/j.canlet.2008.04.053

Figure Lengend Snippet: EndoG expression in human prostate cancer cell lines. (A) Expression of EndoG measured by using real-time RT-PCR. n = 4 per group, *p <0.001 compared to 22Rv1 and <0.05 compared to LNCaP cells. (B) Mn-dependent endonuclease (EndoG) activity in total protein extracts from the cells as measured using plasmid incision assay. O, open circular DNA; L, linear DNA; C, covalently closed supercoiled DNA, D, digested (fragmented) DNA. Each cell extract was tested using two concentrations of protein, 0.6 or 3 μg per sample. (C) Quantification of the plasmid incision assay. n = 4 per group, * p<0.001 compared to 22Rv1 or LNCaP cells.

Article Snippet: Human prostate cancer cell lines, including well-differentiated, 22Rv1 (ATCC # CRL-2505) and LNCaP cells (ATCC # CRL-1740), and poorly differentiated PC-3 cells (ATCC #CRL-1435) were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Plasmid Preparation

Journal: Cancer letters

Article Title: Sensitivity of human prostate cancer cells to chemotherapeutic drugs depends on EndoG expression regulated by promoter methylation

doi: 10.1016/j.canlet.2008.04.053

Figure Lengend Snippet: Sensitivity of prostate cancer cells to cisplatin and etoposide. (A) 22Rv1, LNCaP and PC3 cells were treated with cisplatin (0–100 μM) or (B) etoposide (0–300 μM) for 24 h. Cell death was measured using LDH release assay. n = 4 per group, *p <0.05, **p <0.01, ***p < 0.001 compared to zero point for the same cell line.

Article Snippet: Human prostate cancer cell lines, including well-differentiated, 22Rv1 (ATCC # CRL-2505) and LNCaP cells (ATCC # CRL-1740), and poorly differentiated PC-3 cells (ATCC #CRL-1435) were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Lactate Dehydrogenase Assay

Journal: Cancer letters

Article Title: Sensitivity of human prostate cancer cells to chemotherapeutic drugs depends on EndoG expression regulated by promoter methylation

doi: 10.1016/j.canlet.2008.04.053

Figure Lengend Snippet: Cisplatin-induced death of prostate cancer cells can be prevented by silencing EndoG. (A) EndoG-positive 22Rv1 cells treated with DY547-labeled anti-EndoG siRNA had EndoG expression and DNA fragmentation (TUNEL) significantly decreased compared to the cells treated with transfection reagent only (“non-treated”) or with non-specific control siRNA, and were protected from cell death induced by cisplatin (80 μM) exposure for 24 h. n=4 per group, *p<0.05 compared to non-treated or cells treated with control siRNA. (B) Representative images of the cells after treatment with cisplatin. Bar, 10 μm. (C) Silencing of EndoG in 22Rv1 cells by anti-EndoG siRNA caused increased viability of the cells during exposure with cisplatin (2.5–10 μM) (n=4 per group, *p<0.01 to non-treated or cells treated with control siRNA).

Article Snippet: Human prostate cancer cell lines, including well-differentiated, 22Rv1 (ATCC # CRL-2505) and LNCaP cells (ATCC # CRL-1740), and poorly differentiated PC-3 cells (ATCC #CRL-1435) were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Labeling, Expressing, TUNEL Assay, Transfection, Control

Journal: Cancer letters

Article Title: Sensitivity of human prostate cancer cells to chemotherapeutic drugs depends on EndoG expression regulated by promoter methylation

doi: 10.1016/j.canlet.2008.04.053

Figure Lengend Snippet: Histone acetylation induced by TSA causes EndoG induction and increased sensitivity to cisplatin. (A) Histone acetylation induced by TSA caused EndoG induction only in EndoG-expressing 22Rv1 cells but not in EndoG-negative PC3 cells as determined by Western blotting. The membranes were stained with Ponseau S to control equal protein load (30 μg/well). Histone H4 acetylation was assessed using polyclonal acetyl-histone H4 antibody (Upstate, Lake Placid, NY). (B) Induction of EndoG by TSA (100ng/ml) in 22Rv1 cells was associated with the increased sensitivity to cisplatin (Cis, 25 μM).

Article Snippet: Human prostate cancer cell lines, including well-differentiated, 22Rv1 (ATCC # CRL-2505) and LNCaP cells (ATCC # CRL-1740), and poorly differentiated PC-3 cells (ATCC #CRL-1435) were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Expressing, Western Blot, Staining, Control